that’s not what LD50 means, LD50 is the dose that kills half of the population when exposed to it.
You can’t just go by the dose that causes an obvious effect to estimate the LD50, assuming that you need a higher dose for it to be lethal is a terrible idea.
It is. Unmedicated I can have a full cup of coffee an hour before bed and still sleep like a baby
that’s not what LD50 means, LD50 is the dose that kills half of the population when exposed to it.
You can’t just go by the dose that causes an obvious effect to estimate the LD50, assuming that you need a higher dose for it to be lethal is a terrible idea.